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DEPARTMENT OF BIOLOGICAL SYSTEM PHYSICS
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Cell and DNA Physics
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Study influence of (Lis-Gly), (Ala-Glu-Asp-Gly) and (Ala-Glu-Asp-Leu) bioregulators on chromatin and
nuclei in solutions and chromatin in situ, as well as influence of Cu(II)TOEPyP on DNA; determination of
molecular mechanizm on the base of thermodinamic parameters (ΔHm, ΔSm,
Tm, ΔTm) caused by this interaction.
Influence of a newly Synthesized anticarcinogen TMPy(4) tetramethil pyridil porphirine
analogue Cu(II)TOEPyP(4) on DNA thermodynamic parameters have been studied. It is shown
that the compound preferably binds to GC reach sites, it stabilizes DNA and mild (treatment)
concentrations of it does not cause any harm to the double helix, which is very important in pharmacology.
It is shown that the melting process has entropic and not enthalpic character.
Team leader: J. Monaselidze, Leading Scientist
Team members: M. Gorgoshidze, D. Khachidze, M. Kiladze, E. Lomidze, Sh. Barbakadze
Differential scanning microcalorimetry study of breast cancer and leukemia blood plasma
and erythrocytes thermal characteristics.
Mammary gland duct carcinoma biopsy tissue microcalorimetric investigation has shown that
collagen type IV fibrils have a critical role in stability of epithelia cell matrix. It was determined
that stability of collagen type IV fibrils is decreased by 6 degrees in comparison with norm
Stability of carcinoma epithelia cells is also decreased in comparison to the norm. Namely,
stability of heterochromatin is decreased by 4 degrees, and stability of active chromatin is
decreased by 3 degrees. We offer to use these data for patient diagnosis.
Human leukemia blood plasma calorimetry study has demonstrated that albumin thermostability
in leukemia blood composition is increased by 5-6 degrees in comparison to the norm. These
data are obtained after examination of more than 300 samples. We offer to use these data to
determine the first stage of leukemia.
Calorimeters used in these studies are equipped with software used for calculation of
thermodynamic parameters in solutions and complex biological systems, as well as for data
processing, including deconvulation.
Team leader: J. Monaselidze, Leading Scientist
Team members: M. Gorgoshidze, D. Khachidze, M. Kiladze, E. Lomidze, Sh. Barbakadze, Z. Kuchadze
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